Abstract: To explore flower development molecular mechanism of the Double lily,the AGL6 homologous gene was cloned from Double lily cv. Belonica. The open reading frame of LiAGL6 gene was 744 bp,encoded a protein of 247 amino acids. The LiAGL6 protein molecular weight was 28.3 ku,the grand average of hydropathicity was -0.748 and the theoretical pI was 8.23. The protein structure analysis showed that LiAGL6 protein had a typical MADS-box domain,a K-box region and two AGL6 motifs. Phylogenetic tree analysis showed that LiAGL6 belonged to monocotyledon group,AGL6 branch of the AP1/AGL9 subfamily,and had the highest similarity with Hyacinthus orientalis which was also belonged to monocotyledons, the similarity reached 78%. All the results indicated LiAGL6 was the homologous gene of AGL6, so named it LiAGL6. Cellular localization assay revealed LiAGL6 expressed in the nucleus of onion epidemical cells, which was the basic characteristics of the transcription factors. The qRT-PCR result showed the highest expression of LiAGL6 was in flowers while no expression occurred in leaves and stems. LiAGL6 was expressed most in the seventh whorl petals followed by the sixth and third whorl petals,however,there was almost no expression in the second whorl petals. The research showed the LiAGL6 gene might play a regulatory role on formation of Double lily flowers.

Key words: Double lily, MADS-box, Gene cloning, LiAGL6 gene, Expression analysis

CLC Number: 

• Q78

• S644.03