Abstract: The purpose of this study was to initially analyze the promoter activity and transcriptional regulatory elements of porcine EIF2S3 gene.Using the bioinformatics analysis,PCR amplification,gene clone,cell transfection and dual luciferase activity assay.We obtained the sequence characteristics of the promoter region of EIF2S3,constructed dual luciferase reporter gene vector with different fragment lengths in the EIF2S3 promoter region and analyzed its luciferase activity,identified the core promoter region and key regulatory regions of the EIF2S3,and predicted transcription factors and their binding sites in key regulatory regions.The results showed that the candidate promoter region of EIF2S3 contained 3 core promoters and 2 CpG islands.The -706~+200 bp was the core promoter region and the -706~-253 bp was the key regulatory region of EIF2S3 promoter.This key regulatory region played a positive regulatory role in the transcriptional regulation of EIF2S3 gene.There was no regulatory element that affects the promoter activity in the -1 563~-706 bp of EIF2S3 promoter.The key regulatory region of EIF2S3 promoter contained 440 transcription factor binding sites,and many transcription factors in this region had multiple binding sites,such as SP1,KLF4,MyoD1,MyoG,NFKB1.These data provided a basis for studying the expression mechanism of pig EIF2S3.

Key words: Pig, EIF2S3, Promoter, Transcriptional activity, Transcription factor

CLC Number: 

• S828.2

• Q78