Prediction of Antigens of the Coat Protein of the Strawberry mild yellow edge virus

doi:10.7668/hbnxb.2016.S1.019

Abstract

Abstract: An epitope of antigen is a key part recognized and combined by antibodies. Epitope prediction is helpful to recombinant antigen design and rapid screening,and to preparation of high activity antigen. In this paper,the coat protein of the Strawberry mild yellow edge virus was analyzed and predicted for its secondary structure and physicochemical and biological properties by programs including DNAStar,ExPASy,TMHMM Sever2.0,and IEDB. The results showed that there was no transmembrane regions in the SMYEV coat protein. The average hydrophility of the coat protein was -0.252,which belonged to a soluble protein. There were 5 potential antigen epitopes,of which values of the average antigen index range from 0.026 5 to 0.077 3. Antigen activities of the above predicted epitopes,however,are still to be verified by experiments.

Key words: Strawberry mild yellow edge virus, Coat protein, Epitope, Prediction

CLC Number:

Q78
S668.03

DENG Zibing, MA Jianzhong, WANG Yonggang, YANG Jumei, WEI Yan, PAN Bo, LI Yinwu. Prediction of Antigens of the Coat Protein of the Strawberry mild yellow edge virus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(S1): 106-111. doi: 10.7668/hbnxb.2016.S1.019.

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