Abstract:
The peptides of the linear B-cell epitopes PJ14 and PJ5 on the VP2 protein of infectious bursal disease virus(IBDV)were synthesized and purified,then were conjugated to carrier protein BSA or OVA respectively.The BSA-conjugated PJ14 and PJ5 were inoculated into the BALB/c mice respectively.The OVA-conjugated PJ14 was used as the antigen to detect the anti-PJ14 antibody in the serum of the immunized mice,and so was the OVA-conjugated PJ5.The results showed that the PJ14 and PJ5 after the conjugation to BSA stimulated the production of the anti-PJ14 and anti-PJ5 antibody respectively,and that the antibodies lasted for twenty-one weeks.It indicated the immunogenicity of the PJ14 and PJ5.
Key words:
IBDV,
VP2 protein,
B-cellepitope,
Peptidesynthesis,
Immunization
CLC Number:
XIAO Zhi-jun, ZHANG Gai-ping, YANG Han-chun, YANG Yan-yan, ZHAO Dong, LI Xue-wu, DENG Rui-guang, CHAI Shu-jun, XING Guang-xu, YANG Ji-fei. Mice Immunization with the B-cell Epitopes on the VP2 Protein of Infectious Bursal Disease Virus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(2): 233-235. doi: 10.7668/hbnxb.2010.02.047.