Abstract:
Foot-and-mouth disease virus strain OA/58 RNAs were used as templates for RT-PCR.The amplified cDNA products were cloned into pMD18-T vectors and transformed into JM109.The recombinant plasmids were identified by electrophoresis,PCR,and analysis of tow cleavages with BamH I and Hind III.Depending on homology modeling the FMDV strain OA/58 VP2 protein,the 3D mold was gained.To come out B cell epitopes concerning FMDV OA/58 VP2 protein,several standards were analyzed including physical and chemical characters,hydrophilicity,antigenicity.Many distinct antigenic epitopes in FMDV OA/58 VP2 protein were identified by computation:1-23,40-63,71-78,82-91,102-106,113-119,131-138,148-154,166-177,189-196,212-218.Application of homology molding OA/58 VP2 protein to predict B cell epitopes is reasonable.This method offers reasonable information for researching function of OA/58 VP2 protein,constructing its variant body and selecting new expression forms of OA/58 VP2 protein.
Key words:
Foot-and mouth disease virus,
VP2 protein,
B cell epitope
CLC Number:
ZHOU Jian-hua, CONG Guo-zheng, GAO Shan-dian, CHANG Hui-yun. Construction of VP2 Protein and Prediction of B Cell Epitopes in VP2 from A Foot-and mouth Disease Virus Stra in OA/ 58[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(3): 1-4. doi: 10.7668/hbnxb.2008.03.001.