Cloning,Sequencing and B Cell Epitopes Prediction of Structural Protein Gene of a Foot-and-Mouth Disease Virus Strain Asia1/HeB

doi:10.7668/hbnxb.2009.04.012

Abstract

Abstract: The DNA of P1 gene of Foo-t and-mouth disease virus Asia1/HeB was amplified and cloned, and then sequenced. Amino acid sequence of P1 on secondary structure, flexibility, hydrophilicity, accessibility and ant igenicity were analyzed using DNAStar Protean software,with the B cell epitopes to be predicted.The results showed that the P1 region of FMDV Asia1/HeB is 2 199 bp in length and codes for a polypeptide of 733 amino acids, including complete open reading frame. The structural protein of VP1,VP2,VP3,VP4 gene consists of 633, 654, 657, 255 bp and codes for 211, 218, 219, 85 amino acids respect ively. The secondary structure of P1 region was relatively complicated consisting of more B sheet regions and turn regions.There were predominant B cell epitopes in VP1,VP2, VP3 and a small amounts in VP4. Comparing with the epitopes that have been published, the predict results have a high reliability. This study would be helpful for identification of B cell epitopes for structural protein of FMDV Asia1/HeB strain using experimental methods and research of reverse vaccine of FMDV.

Key words: Foo-t and-mouth disease virus, Structural protein, Sequencing, B cell epitopes

CLC Number:

ZHANG Zhong-wang, ZHANG Yong-guang, WANG Yong-lu, PAN Li, FANG Yu-zhen, LIU Li-kuan, JIANG Shou-tian, LU Jian-liang, ZHOU Peng, ZHANG Yu, ZHANG Shu-gang, DU Jin-xin, LI Zheng-feng. Cloning,Sequencing and B Cell Epitopes Prediction of Structural Protein Gene of a Foot-and-Mouth Disease Virus Strain Asia1/HeB[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(4): 57-63. doi: 10.7668/hbnxb.2009.04.012.

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