Effects of Crop Rotation with Legumes on the Composition of Microbial Community in Greenhouse Vegetable Soils

doi:10.7668/hbnxb.20191958

Abstract

Abstract: The crop rotation system adjustment based on nutrient reduction of legumes is helpful to realize the prevention of agricultural non-point source pollution on the basis of ensuring high yield. To investigate the effect of different rotation systems on microbial communities, soil samples were collected from three rotation systems, i.e. tomato-melon, beans-melon and tomato-beans in location test. MiSeq pyrosequencing and soil properties analysis were conducted to analyze the differences in the diversity, composition and structure of microbial communities in soils under legume based rotations compared with those of traditional Solanaceae-Cucurbitaceae rotation and to determine the controlling factors of the variation in microbial communities. Results showed that the bacterial Pielou index and fungal Chao index were significantly reduced in soils of bean-melon soils and tomato-beans, respectively. The relative abundances of Rokubacteria (Bacterial phylum) and Basidiomycota (fungal phylum) were significantly increased by 2.3 and 4.1-4.8 times, respectively, in soils of tomato-beans compared to that in the other two systems. At family level, the relative abundances of Nitrosomonadaceae,Rokubacteriales and S085 (bacteria), and Plectosphaerellaceae,Cordycipitaceae and Nectriaceae (fungi) was significantly increased in soil of tomato-beans. The relative abundance of Steroidobacteraceae was significantly increased in soil of bean-melon, while the relative abundances of Roseiflexaceae and Hyphomonadaceae were promoted in soil of tomato-melon. Canonical correlation analysis (CCA) indicated that changes in the microbial community composition were strongly influenced by vegetable types, soil pH and NO₃^--N content. Moreover, we found that bacteria was more sensitive than fungi in response to changes in soil variables. Collectively, the present study showed that the adjustment of the rotation system by introducing legumes had significant impact on the soil microbial community by decreasing microbial diversity and altering community composition. Soil bacterial community responded more sensitively to the alteration of rotation system than fungi in greenhouse soils.

Key words: Microbial community, Greenhouse soil, Legume, Rotation, High-throughput pyrosequencing

CLC Number:

S154.3

LIU Lei, XU Meng, WANG Ling, RU Shuhua, SUN Shiyou, GAO Jing, LI Pin, ZHANG Guoyin, MA Junxian. Effects of Crop Rotation with Legumes on the Composition of Microbial Community in Greenhouse Vegetable Soils[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 203-215. doi: 10.7668/hbnxb.20191958.

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