Cloning and Molecular Characteristics of SRY Gene in Yak

doi:10.7668/hbnxb.2009.04.011

Abstract

Abstract: SRY (Sex2determining Region on the Y Chromosome,SRY) gene is located on Y chromosome of mammal and plays a key role of sex determination.Aiming at discovering the mechanismof sex determination in yak,the SRY gene was cloned and its Bioinformatics characterization was analyzed. Taking the blood of Plateau yak as material,the SRY gene was amplified from Yak′s genome. The product was cloned to pGEM2T easy vector and was sequenced.At the same time,the coding region of SRY gene of yak was compared to cow′s. The SRY protein of yak and other species′was aligned. Through bioinformatics software,the characteristics and structure was predicted for the SRY protein of yak. The total coding region of Yak′s SRY gene ( GenBank : EU547257) is 687 bp, encoding a peptide with 229 amino acid residues. Through sequences aligned,it was discovered that there are 2 mutations in the coding protein of yak. There is high homology among different species. The SRYprotein of yak is mainly constructed by hydrophilicity amino acids. Ho2 mogenous 3D model of HMGof SRYobtained by predicting shows that HMGof SRY including three Alpha helixes,e2 merging ″L″shape. The coding region of SRY gene in Plateau yak was cloned for the first time and its molecular charac2 teristics was shown. This work laid an important foundation for controlling sex formation of yak.

Key words: Yak, SRY gene, Clone, Molecular characteristics

CLC Number:

PEI Jie, YAN Ping, CHENG Sheng-li, CHU Min, FENG Rui-lin, LIANG Chun-nian, GUO Xian, ZENG Yu-feng, BAO Peng-jia, ZHU Xin-shu. Cloning and Molecular Characteristics of SRY Gene in Yak[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(4): 50-56. doi: 10.7668/hbnxb.2009.04.011.

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