Molecular Evolution Character of Complete Coding Sequence of Bovine Myostatin Gene

doi:10.7668/hbnxb.2009.04.010

Abstract

Abstract: We obtained complete coding sequence of myostatin gene from Bos taurus, Bos indicus, Bos grunniens, Bos f rontalis and Bubalus bubalis (swamp buffalo and river buffalo) by PCR fragments sequencing method. The molecular evolution characters of CDS of myostatin gene were analyzed. The result showed that CDS of bovine MSTN gene was 1 128 bp in length. The mutation rate within species was between 0 and 0. 35 %,while the mutation rate between species was between 0. 08 % and 1. 42 %. The codon usage bias phenomenon arosed and the 29 preferenced codon possessed about 49. 2 % of the total preferenced codons. The predominant substitution model of nucleotide was transition and the ratio of transition and transversion was 2. 9. Nonsynonymous sites were far less than synonymous sites. The ratio of synonymous and nonsynonymous substitution were all less or equal to one manifesting that it did not effected by Darwin positive selec2 tion.But some sites affected by negative selection.Molecular tree showed that haplotypes from Bubalus bubalis and Bos indicus clustered on their own and there were apparent differentiation between Bubalus bubalis, Bos indicus and other bovine species. There was no apparent differentiation among Bos taurus,Bos grunniens,Bos f rontalis and they maybe pro2 duced apparent differentiation gradually in the process of evolution.

Key words: Bovine species, Myostatin gene, Complete coding sequence, Molecular evolution

CLC Number:

WANG Lan-ping, GENG Rong-qing, JI De-jun, CHANG Hong, CHANG Chun-fang, LI Yong-hong. Molecular Evolution Character of Complete Coding Sequence of Bovine Myostatin Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(4): 46-49. doi: 10.7668/hbnxb.2009.04.010.

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