Effects of Chinese Yam Polysaccharide on Antibody and T Lymphocytes Subpopulations in the Immune Response to PRRSV Killed Vaccine

doi:10.7668/hbnxb.2010.02.048

Abstract

Abstract: The research is to study the effects of Chinese yam polysaccharide(CYPS)on antibody and T subpopulations in piglets pheripheral blood in the immune response to PRRSV killed virus vaccine.16 35-day-old piglets were randomly assigned into equal four groups and three groups were vaccinated with PRRSV killed virus vaccine.At the same time,the piglets in groups CYPSH and CYPSL were intramuscularly injected with CYPS at 17.1 or 8.55 mg/kg dosages,group C(control group),with sterile saline,once a day for three successive days,group F(fake group)only injected with saline.At 7,14,24,34,44,54,69,79 day after vaccination,pheripheral blood were sampled for detection of serum antibody titer to PRRSV by ELISA and T lymphocytes subpopulations by cytofluorometry.Results showed that CYPS can boost the proportion of CD3⁺ and CD8⁺ lymphocytes in piglets pheripheral blood after immunization with PRRSV killed virus vaccine,and enhanced antibody titer to PRRSV after 34 days.CYPS is a potential immunostimulatory for PRRSV killed virus vaccine.

Key words: CYPS, PRRSV killed virus vaccine, Antibody, T lymphocyte subpopulations

CLC Number:

S852.65

ZHANG Hong-ying, WANG Xue-bing, CUI Bao-an, ZHAO Xian-min, CHEN Hong-ying, JIN Yue. Effects of Chinese Yam Polysaccharide on Antibody and T Lymphocytes Subpopulations in the Immune Response to PRRSV Killed Vaccine[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(2): 236-238. doi: 10.7668/hbnxb.2010.02.048.

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http://www.hbnxb.net/EN/Y2010/V25/I2/236

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