The Construction of Hybridomas Secreting Monoclonal Antibodies against Infectious Bursal Disease Virus in Chicken and Its Application

doi:10.3321/j.issn:1000-7091.1990.01.019

Abstract

Abstract: BALB/C mice were immunized with infectious bursal disease virus(IBDV) which had been purified with ammonium sulfate precipitation，high一speed centrifugation and ultracentrifugation In a sucrose gradient.From the fusion of myeloma cells NS一1 with immun spleen cells，11 strains of positive hybridoma cells occurred.After cloning three times and ELISA test, three strains of hybridomas(1B_t,5D₆，6D₈) were selecten which were able to Produce monoclonal antibodies to the IBDV continuously.Cell supernatant valences are 1B₁, 3.2 ×10₂;5D₄,6.4×10₂₈, 8×10₂; Ascites fluid valances are 1B₁, 1.6 ×10₅;5D₈,8×10₄₈, 3.2×10₃; Their chro-mosome are 1B₁,105, 5D₈,97 and 6D₈,85.IBDV McAbs didn't cross-react with ND，MD and IA antigens.Their immunogobulin class/subclas.s are all IgG₁.They have different antigenic determinants and all show a characteri-stic of precipitation。The primary application of the indirect ELISA inhi-biting assay for detection of IBDV antibodies in chicken is investigated.

Key words: ELISA, Monoclonal antibody, Infectious bursal disease virus (IBDV)

Qiao Zhong, Zhan Li’e, Ning Guanbao, Shi Zhenxin. The Construction of Hybridomas Secreting Monoclonal Antibodies against Infectious Bursal Disease Virus in Chicken and Its Application[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1990, 5(1): 98-104. doi: 10.3321/j.issn:1000-7091.1990.01.019.

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