Abstract: The construction of molecular cloning,antigenicity identification and bioinformatics analysis of Vibrio alginolyticus outer membrance protein U(OmpU),laid the foundation for the development vaccine.The OmpU expression strain was obtained by molecular clone.OmpU was purified by the way of the gel slices,mice immunized were to prepare antiserum and found that OmpU had better antigenicity using Western Blotting method.By online software,the prediction results showed that OmpU protein was a hydrophilic and secreted protein multiple restriction enzyme sites.OmpU secondary structure contained random coil 27.94%,alpha helix 33.24%,beta turn 10.29%,β sheet 28.53% by SOPMA method.Using TMHMM Server v.2.0 software predicted that OmpU had not transmembrane structure and was located outside cell membrane,and the SignalP 4.1 software found that the 1-21 amino acids of OmpU was signal peptide sequence.The 3D structure prediction found that OmpU protein had 3 core structure.Using ABCpred and BepiPred prediction program,OmpU had 8 B cell epitopes.By the prediction method of artificial neural network,OmpU protein had 3 CTL epitopes.OmpU protein had 1 Th epitopes using an online server prediction for MHC class Ⅱ peptide binding affinity.

Key words: Vibrio alginolyticus, OmpU protein, Cell epitopes, Protein structure, Prediction

CLC Number: 

• S942

• Q78