Abstract: There are many morphological and physiological changes during ontogeny in higher plants.For the better understanding of the proteomic differences between ontogenetic phases,according to researching the main developmental stages of sporogonium and microsporocyte in cultivars 49 of Eustoma grandiflorum by using fluorescent two-dimensional difference gel electrophoresis (2D-DIGE) carried out proteome differential expression,analysing the functions of the differential proteomics during the different periods of stamens development.The result showed that 72 differentially expressed or modified protein spots were separated by software named DeCyder.While the amount of protein increased in 35 spots during ontogeny and declined in 37 spots.There were fifteen spots whose credibility were as high as 90% had been successfully picked and identified by MALDI-TOF-TOF high-resolution tandem mass spectrometry.Names were 5-methyl 4 hydrogen adjustment acyl glutamic acid homocysteine three methyl transferase,S-adenosine methionine synthetase,33 kDa light system Ⅱ oxygen complexes,1,5-ribulose bishosphate carboxylase,plastid division ring protein,mitochondrial outer membrane protein film hole 34 kDa,heat shock protein 70 kDa,phosphoglycerate kinase,proteolytic enzyme complex beta 6 type subunits,aspartic protease,The ATP and Zn²⁺ dependent metalloproteinases and unknown function protein.Therefore,the differential proteomics expression makes the morphological and physiological changes of the different periods of stamens development.

Key words: 2D-DIGE, Differential proteomics, Eustoma grandiflorum, Sporogonium, Microsporocyte

CLC Number: 

• Q78