Differencial Expression of Fertility-related Proteins in Chinese Cabbage

doi:10.7668/hbnxb.2015.02.023

Abstract

Abstract: To better understand the molecular mechanisms of multiple-allele-inherited male sterility in Chinese cabbage (Brassica rapa L.ssp pekinensis), the iTRAQ quantitation technique based on mass spectrometry were used to study the differencial expression between proteins fertile- and sterile-buds.This research would provide the basis for analyzing the molecular mechanism of multiple-allele-inherited male sterility in Chinese cabbage.In this research, a total of 358 differencial expressed proteins (226 up-expressed and 132 down-expressed proteins in fertile buds) were found and Gene Ontology (GO) analysis showed that the proteins identified data has better coverage of biological functions.It is verified by two-dimensional electrophoresis (2-DE).This results is consistent with iTRAQ technique, which indicated that using iTRAQ to analyze protein profile is reliable.

Key words: Chinese cabbage, Male sterility, iTRAQ, Differential proteomics

CLC Number:

Q78
S634.03

BI Qing, ZHOU Xue, JI Rui-qin, FENG Hui. Differencial Expression of Fertility-related Proteins in Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(2): 128-131. doi: 10.7668/hbnxb.2015.02.023.

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