Abstract: The objective of this study is to clone and analyze the sequence characterization of strawberry mild yellow edge virus CP gene, then construct its prokaryotic expression vector and express its protein in Escherichia coli Rosetta-gami B(DE3). Virus detection primers was used to detect the strawberry plantlets infected with SMYEV by RT-PCR, and a pair of primers were designed according to the uploaded sequence of SMYEV CP gene in NCBI to isolate the gene of SMYEV isolated in Shenyang region(SY05). The CP gene of SY05 was subcloned into expression vector pGEX-6p-1 and then transferred into Escherichia coli Rosetta-gami B(DE3) pLysS, finally this CP protein was induced by IPTG to express. Sequence analysis indicates that the SMYEV CP gene from SY05 is 729 bp in length and encodes 242 amino acid residues. The nucleotide sequence of this CP gene shares 80. 1%-97. 4% homology with other CP genes of SMYEV isolated from different plants. The deduced protein shares 92. 1%-99. 6% with the other homologous genes, including a 98. 3% homology with that isolated from SY03. The CP gene was subcloned into the expression vector pGEX-6P-1 and transferred into prokaryotic cells. Induced with IPTG, a 52. 0 kDa induction expression band of fusion protein was found in SDS-PAGE detection. All the results showed that we have isolated the CP gene of SMYEV in SY05 and prokaryotic expression of this gene was realized. This research provides a foundation for the serology research of SMYEV detection in strawberry.

Key words: Strawberry mild yellow edge virus, Coat protein gene, Prokaryotic expression

CLC Number: 

• Q78