Comparative Studies on Proteomic Altered in Pollen Mother Cells(PMC) of Cytoplasmic Male Sterility and Corresponding Maintainer Line in Cauliflowers

doi:10.7668/hbnxb.2016.S1.018

Abstract

Abstract: In order to investigate the mechanism of cytoplasmic male sterility in Cauliflower,we analyzed the proteomic altered with pollen mother cells(PMC) at different developmental stages(meiosis stage of PMC,double-three nucleus stage and pollen grain stage) of cytoplasmic male sterility line(CMS) and corresponding maintainer line in cauliflowers. The results showed that proteomic altered was observed in CMS compared with maintainer line,especially in double-three nucleus stage. 4 protein spots(spot 1,9,19,20) were lost and 7 protein spots were showed decreased and 10 protein spots were increased in protein abundance in CMS,respectively,10 protein spots were identified using MS/MS and NCBI database searching. The results demonstrated that these proteins were involved in a wide range of signal transduction,energy metabolism,protein biosynthesis,photosynthesis and resistance reaction. These experimental results may be used in male sterility mechanism investigation and maintainer line selection in cauliflowers.

Key words: Cytoplasmic male sterility, Proteomic, Double-three nucleus, MS/MS, Cauliflower

CLC Number:

Q78
S635.03

BIAN Lina, FAN Baoli, SUN Deling, PENG Yongkang. Comparative Studies on Proteomic Altered in Pollen Mother Cells(PMC) of Cytoplasmic Male Sterility and Corresponding Maintainer Line in Cauliflowers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(S1): 101-105. doi: 10.7668/hbnxb.2016.S1.018.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2016.S1.018

http://www.hbnxb.net/EN/Y2016/V31/IS1/101

References

[1] 郭晓芹,仪泽会,惠麦侠,等.大白菜——花椰菜异源多倍体的形态、细胞及分子鉴定[J].园艺学报,2012,39(7):1365-1372.
[2] 朱惠霞,陶兴林,胡立敏.花椰菜细胞质雄性不育系的细胞学研究[J].中国蔬菜,2011(18):64-67.
[3] 顾爱侠,申书兴,陈学平.大白菜与结球甘蓝异源三倍体的获得及细胞学观察[J].园艺学报,2006,33(1):73-77.
[4] 楼珏,张小玲,罗天宽,等.利用SSR和SRAP标记分析花椰菜自交系的遗传多样性[J].分子植物育种,2015,13(3):605-614.
[5] 荆赞革,唐征,裴徐梨,等.花椰菜种质资源遗传多样性SRAP标记分析[J].中国农学通报,2014,30(7):64-69.
[6] 马二磊,王燕,刘莉,等.松花型花椰菜主要品种鉴定的分子标记分析[J].植物遗传资源学报,2010,11(5):621-624.
[7] 王桂香,严红,曾兴莹,等.花椰菜——黑芥体细胞杂交获得抗黑腐病异附加系新材料[J].园艺学报,2011,38(10):1901-1910.
[8] Dey S S,Sharma K,Dey R B,et al. Inter specific hybridization(Brassica carinata×Brassica oleracea)for introgression of black rot resistance genes into Indian cauliflower(B-oleracea var. botrytis L.)[J]. Euphytica,2015,204(1):149-162.
[9] 吕晶,刘凡,宗梅,等.花椰菜-黑芥渐渗系和异附加系的获得与分析[J].园艺学报,2014,41(3):456-468.
[10] 胡立敏,陶兴林,朱惠霞,等.花椰菜新品种圣雪3号的选育[J].中国蔬菜,2015(1):55-56.
[11] 牛国保,姚星伟,孙德岭,等.花椰菜新品种春雪1号的选育[J].中国蔬菜,2015(4):67-68.
[12] 胡立敏,陶兴林,朱惠霞,等.花椰菜新品种圣雪4号[J].园艺学报,2015,42(5):1007-1008.
[13] Ahmad R,Khan R S,Farhatullah. Transcript analyses of the gene associated with CMS in Brassica napus[J]. Euphytica,2014,200(3):455-463.
[14] 王春国,陈小强,李慧,等.花椰菜细胞质雄性不育系及保持系中特异序列的克隆、分析[J].分子细胞生物学报,2008,41(1):19-27.
[15] Wang C G,Chen X Q,Lan T Y,et al. Cloning and transcript analyses of the chimeric gene associated with cytoplasmic male sterility in cauliflower(Brassica oleracea var. botrytis)[J]. Euphytica,2006,151(1):111-119.
[16] Yan S,Tang Z,Su W,et al. Proteomic analysis of salt stress-responsive proteins in rice root[J]. Proteomics,2005,5(1):235-244.
[17] Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry,1976,72:248-254.
[18] Laemmli U K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4[J]. Nature,1970,227(5259):680-685.
[19] 范昌发,孙春昀,郭骁才,等.细胞质雄性不育高粱叶绿体ndhD基因的序列变异[J].遗传学报,2002,29(10):907-914.
[20] 杨景华,张明方,喻景权,等.叶用芥菜细胞质雄性不育相关基因 orf 220 的分子特性[J].遗传学报,2005,32(6):594-599.
[21] 易平,汪莉,孙清萍,等.红莲型细胞质雄性不育线粒体相关嵌合基因的发现[J].科学通报,2002,47(2):130-133.
[22] Ogura H. Studies on the new male-sterility in Japanese radish,with special references to the utilization of this sterility towards the practical raising of hybrid seeds[J]. Mem Fac Agric Kaghshima Univ,1968,6:39-78.
[23] Ivanov M K,Revenko A S,Dymshits G M. Cytoplasmic male Sterility-associated structural variation of the mitochondrial genome regions containing rps3 and orf215 in sugar beet Beta vulgaris L.[J]. Molecular Biology,2004,38(3):345-350.
[24] 吴智明,胡开林,陈晓莹,等.用AFLP技术分离辣椒mtDNA中与雄性不育相关的基因片段[J].核农学报,2010,24(1):20-24.

Please choose a citation manager

Content to export