Effects of Nitrogen Application for Bud Development on Protein Expression of Flag Leaf at Late Grain Filling Stage in the First Cropping Rice

doi:10.7668/hbnxb.2016.03.019

Abstract

Abstract: By using comparative proteomics combined with bioinformatics and related physiological indicators,this paper studied the regulating effects of nitrogen application for bud development on protein expression of flag leaf at late grain filling stage in the first cropping rice with ⅡYou 1273 as a material.Two treatments of urea levels:300.00,0.00 kg/ha applying at 18 d after heading stage were included in the experiment in the first cropping rice.A total of 15 differentially expressed proteins were detected in different fertilizer treatments by the 2D maps of flag leaf proteome.And these proteins were grouped into three expression patterns according to their functions in photosynthesis carbon assimilation,electron-transfer and photophosphorylation,the defense response to stresses etc..Our results indicated that the proteins involving photosynthesis were down-regulated at late grain filling stage in the first cropping rice.But,nitrogen application for bud development could significantly slow down the decreased amplitude of photosynthesis-related proteins in expression abundance,which relatively enhanced the chlorophyll content and photosynthetic rate of functional leaf and increased the source supply ability at late grain filling stage in the first cropping rice.The result also showed that nitrogen application for bud development could significantly increase the expression abundance of resistance-related protein at late grain filling stage in the first cropping rice,which helped enhance the scavenging of active oxygen,retard the peroxidation of membrane lipid and delay the senescence rate of flag leaf.As a result,the seed-setting rate and yield of the first cropping rice were significantly increased under NA treatment.

Key words: Ratooning rice, Leaf, Nitrogen application for bud development, Proteomics, Grain-yield

CLC Number:

S511.03

CHEN Hongfei, ZHANG Zhixing, LIN Wenxiong. Effects of Nitrogen Application for Bud Development on Protein Expression of Flag Leaf at Late Grain Filling Stage in the First Cropping Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(3): 127-134. doi: 10.7668/hbnxb.2016.03.019.

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