Cloning and T Cell Epitopes Prediction of Structural Protein Gene of a Foot-and-Mouth Disease Virus Strain AF/72

doi:10.7668/hbnxb.2010.05.006

Abstract

Abstract: Virus strain AF/72 RNAs were used as templates for RT-PCR. The amplified cDNA products were cloned into pGEM-T easy vectors and transformed into JM109. The recombinant plasmids were identified by electrophoresis,PCR,and EcoR I cleavage. Application of biological software to H-2Dv,H-2Kv,H-2Lv,A20 restricted T cell epitope and the difference of restrictive T-cell epitope were compared. The results showed that the VP1 region of FMDV AF /72 strain was 639 bp in length and codes for a polypeptide of 213 amino acids. Although different restrictive T cell epitope was not identical,but the predicted T cell epitope was similar to compared with identified T cells and might is the universal T-cell. This study prediction of T cell epitope of structural protein of FMDV AF /72 strain through bioinformatics. It would be helpful for identification of T cell epitopes for structural protein of FMDV AF /72 strain using experimental methods and provides an effective method for the virus T cell epitope research simultaneously,for the virus epitope based accines design provides effective theoretical basis.

Key words: FMDV, T cell epitope, VP1 protein

CLC Number:

LIU Xinsheng, WANG Yonglu. Cloning and T Cell Epitopes Prediction of Structural Protein Gene of a Foot-and-Mouth Disease Virus Strain AF/72[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(5): 28-32. doi: 10.7668/hbnxb.2010.05.006.

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