Prokaryotic Expression and Activity Analysis of VP1 Gene of Foot-and-Mouth Disease Virus Serotype O

doi:10.7668/hbnxb.2009.06.003

Abstract

Abstract: The objective of this research is to get the FMDV VP1 protein which be of antigenancy activity.The recombinant expression vector pET2VP1 was constructed by cloning VP1 gene of FMDV into the prokaryotic expression plasmid pET32a from PMD18T2VP1.After the recombinant plasmid was verified by PCR and sequenced analysis,the result showed that VP1 gene was successfully cloned into expression plasmid pET32a,The protein corresponding to the VP1 gene was expressed after induction with IPTG.SDS2PAGE and Western2blot with the product of bacterial culture in different time showed that the molecular mass of the protein was approximately 45 ku,which was identified by positive sera of FMDV;The protein was refolded by dialysis method,and was assayed by ELISA,the result showed that it demonstrates high activity.So we can say that VP1 protein was expressed efficiently in E.coli,and the renaturation VP1 protein can be developed as diagnosis antigen or vaccine.

Key words: FMDV, VP1 gene, Prokaryotic expression, Protein renaturation

CLC Number:

Q786

YANG Su-zhen, ZHANG Gai-ping, BAO Deng-ke, QIAO Song-lin, WAN Bo, FAN Jian-ming, ZHI Ai-min, LI Xue-wu. Prokaryotic Expression and Activity Analysis of VP1 Gene of Foot-and-Mouth Disease Virus Serotype O[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(6): 11-14. doi: 10.7668/hbnxb.2009.06.003.

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