Bioinformatic Analysis of the auxin Gene Family in Wheat and Rice

doi:10.7668/hbnxb.2009.06.004

Abstract

Abstract: One of the sequenced 260 bp cDNAfragments was highly identified with auxin.The ESTwas extended using CAP3 software in the lab local net.Based on the extended sequence,a pair of primers was designed in the two side regions of its predicted sequence and a cDNA fragment of 608 bp was isolated from Mardler/ 7 3 Bainong 3217 using RTPCR method.The sequence encoded a peptide of 20deduced amino acid residues.The accession numbers were AY902381(gene) and (protein).The deduced molecular weight of protein was 23.0 kDa and the theoretical pI was 9.93.We used the Auxin response domain sequences identified from the auxin genes in plants as queries to search for auxin genes in both wheat and rice,and a polygenetic tree was constructed based on the corresponding auxin proteinsfromwheat and rice.The cell location and protein structure similarityof these genes were analyzed by searching the EST sequences of these genes,and it is essential to study this gene further based on the function of known similar genes.

Key words: Wheat, Rice, Auxin

CLC Number:

Q811

ZHANG Jun-hong, MENG Cheng-sheng, ZHANG Cai-ying, SHI Zheng, WANG Xiao-ying, LI Ai-li, MA Zhi-ying. Bioinformatic Analysis of the auxin Gene Family in Wheat and Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(6): 15-19. doi: 10.7668/hbnxb.2009.06.004.

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