Molecular Cloning and Characteristics of cDNA Encoding Pig β1 Subunit for FMDV Receptor

doi:10.7668/hbnxb.2007.06.004

Abstract

Abstract: In this study, we first molecularly cloned pig integrin β1 from RNA of the tongue and lung of recovered pig infected experunentally with foot-and-mouth-disease virus(FMDV), and compared with the β1 gene of other animals available in GenBank at nucleotide and amino acid loves. The 2397 bp cDNA of pig irnegrln β1 encodes a polypeptide of 798 amino acids consisting of 10 potential N-linked glycosylation sites(NXTX/NXSX), 2 EGF-like domains and 3 cys-teine-rich regions. Pig integnin β1 aubunit has a 20-residue putative signal peptide, a 708-residue ectodomain, a 29-residue transmembrane domain, and a 41-residue cytoplasmic domain.The nucleotide sequence similarity of integrin βl between bovine, chimpanzee, cat, dog, human, mouse, chicken is 99.5%, 90.0%, 91.8%, 90.7%, 90.2%, 86.5% and 77.4%, and the amino acid sequence similarity is 99.9%, 93.9%, 97.5%, 96.7%, 94.2%, 92.4% and 94. 9% respectively.Hydrophobicity analysis of the polypepticle revealed two hydrophobic domains, the signal peptide(1-20AA)and transmembrane domain(729-757AA).This study will lay a foundation for understarxling the interactions of FMDV with recptors.

Key words: FMDV, Receptor, Pig integrin β1 cDNA, Molecular characteristics

CLC Number:

S852.65
Q78

GAO Shan-dian, DU Jun-zheng, CHANG Hui-yun, ZHOU Jian-hua, XIE Qing-ge. Molecular Cloning and Characteristics of cDNA Encoding Pig β1 Subunit for FMDV Receptor[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(6): 14-18. doi: 10.7668/hbnxb.2007.06.004.

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