Characterization,Transcript Variants of Growth Hormone Secretagogue Receptors 2( GHSR2S) Gene and Tissue Expression of GHSRs in Cyprinus carpio var. jian

doi:10.7668/hbnxb.2013.05.002

Abstract

Abstract: Growth hormone secretagogue receptors ( GHSRs ), the endogenous receptor of growth hormone se-cretion ( ghrelin),belong to G-protein-coupled receptors family,and play a regulatory role on animal growth,energy homeostasis etc.In this experiment,RT-PCR and PCR methods were applied to isolate two jlGHSR2s,jlGHSR2a and 2b,eccoding 366 and 367 amino acids sharing 98% identity with each other,and 95% identity with Danio rerio GHSR2.Intron of jlGHSR2s is located at 793 bp after start codon,there are obvious differences in length and se-quences between 2a and 2b introns.We first report jlGHSR2a',the variant of jlGHSR2a,which is selectively spliced last 57 bp of exon 1 and reserved last 28 bp of intron near exon 2.jlGHSR2a'encodes 350 amnio acids,missing the last two transmembrane region,and two ends of splicing sequence are CT-AC.Fluorescent quantitative RT-PCR re-sults showed that the highest expression level of jlGHSRs is in brain and testis,1s is expressed in all the tissues ex-amined in this experiment,but2 s is rarely expressed in other tissues. The expression of variants 1 s'and 2 a'has cer-tain degree of positive correlation with 1s and 2s,but 1s/1s'is inconsistent among these tissues,the highest ratio is Growth hormone secretagogue receptors ( GHSRs ), the endogenous receptor of growth hormone se-cretion ( ghrelin),belong to G-protein-coupled receptors family,and play a regulatory role on animal growth,energy homeostasis etc.In this experiment,RT-PCR and PCR methods were applied to isolate two jlGHSR2s,jlGHSR2a and 2b,eccoding 366 and 367 amino acids sharing 98% identity with each other,and 95% identity with Danio rerio GHSR2.Intron of jlGHSR2s is located at 793 bp after start codon,there are obvious differences in length and se-quences between 2a and 2b introns.We first report jlGHSR2a',the variant of jlGHSR2a,which is selectively spliced last 57 bp of exon 1 and reserved last 28 bp of intron near exon 2.jlGHSR2a'encodes 350 amnio acids,missing the last two transmembrane region,and two ends of splicing sequence are CT-AC.Fluorescent quantitative RT-PCR re-sults showed that the highest expression level of jlGHSRs is in brain and testis,1s is expressed in all the tissues ex-amined in this experiment,but2 s is rarely expressed in other tissues. The expression of variants 1 s'and 2 a'has cer-tain degree of positive correlation with 1s and 2s,but 1s/1s'is inconsistent among these tissues,the highest ratio is in the liver,followed by muscle,intestine and brain,testis and ovary are the minimum ones. Growth does no signifi-cant effect on the jlGHS R1s expression in brain,but jlGHSR1s expression in liver and intestine of slow growth is higher than fast growth ones, 1s/1s'is also increased obviously.It is opposite in muscle,1s/1s'in male muscle is obviously decreased.The growth speed or starvation could do no significantly effects on jlGHSR2 s expression in the brain.Our results show that the jlGHSR1s expression level is clearly correlated with growth.

Key words: Cyprinus carpio var. jian, Growth hormone secretagogue-receptor, Transcript variants, Expression

CLC Number:

S917.4

LI Hong-xia, LI Jian-lin, TANG Yong-kai, YU Ju-hua, RUAN Rui-xia, WEI Ke-peng, XIA Zheng-long, DONG Zai-jie. Characterization,Transcript Variants of Growth Hormone Secretagogue Receptors 2( GHSR2S) Gene and Tissue Expression of GHSRs in Cyprinus carpio var. jian[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(5): 6-14. doi: 10.7668/hbnxb.2013.05.002.

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