Abstract: A novel 14-3-3 gene from Ganoderma lucidum was cloned in silico based on the corresponding Gan-oderma lucidum EST sequences in NCBI database. Some characters of amino acids encoded by 14-3-3 gene,inclu-ding the composition of amino acid sequence,physical and chemical properties,transmembrane domain,hydropho-bicity /hydrophilicity,subcellular localization,secondary and tertiary structure of protein plus the functional domain, were analyzed by bioinformatics tools. A 14-3-3 gene from Ganoderma lucidum was 1 372 bp and it contained a complete ORF which encoded 257 amino acids localized in the cytoplasm,there was not signal peptide and trans-membrane helical regions,was not a secreted protein. The secondary structure were composed of 6.23% random coil,66.54% α-helix and the 27.24% extension chain.Homology comparison and phylogenetic analysis showed that the amino acid encoded by 14-3-3 gene in Ganoderma lucidum was highly homologous with those encoded by 14-3-3 gene in mushroom species. As the real-time PCR results showed,the gene transcription expression level un-der the liquid static cultivation was obvious higher than that in liquid submerged cultivation.

Key words: Ganoderma lucidum, 14-3-3 protein, In silico cloning, Bioinformatics, Expression

CLC Number: 

• S567.31