Modeling and Analysis of 3C Protease Structure and Function from A Foot-and-month Disease Virus Strain OA/58

doi:10.7668/hbnxb.2007.06.003

Abstract

Abstract: Foot-and-mouth disease virus strain OA/58 RNAs were used as templates for RT-PCR.The amplified cDNA products were cloned into pGEM-T Easy Vectors and transformed into JM 109. The recombinant plasmids were identified by electrophoresis, PCR, and Eco R I cleavage. The nucleotide and amino acid sequences were compared with the 3C sequences of poliovirus, Hepatitis A virus and Human rhinovirus 89. The sequences alignments revealed that the agreement of OA/ 58 3C sequence to three reference viruses are 38.8%, 37. 1%, 36.5% at the nucleotide level, 23. 7%, 19. 2%, 17.6% at the acid level. By means of Swiss-pdbV iewer software, the 3D and surface mold of FMDV OA/58 3C protease was ohtained. In addition, Cys₃₁-His₄₆-Asp₈₄ may he an active site in FMDV、OA/58 3C protease.

Key words: FMDV, 3C protease, Active site

CLC Number:

S852.65
Q78

ZHOU Jian-hua, CONG Guo-zheng, GAO Shan-dian, CHANG Hui-yun. Modeling and Analysis of 3C Protease Structure and Function from A Foot-and-month Disease Virus Strain OA/58[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(6): 9-13. doi: 10.7668/hbnxb.2007.06.003.

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