Screening and Identification of B Cell Epitopes of VP1 of Foot-and-mouth Disease Virus

doi:10.7668/hbnxb.2009.05.018

Abstract

Abstract: Foot-and-mouth disease virus strain AF72 RNAs were used as templates for RT-PCR to amplify the VP1 gene.The purified PCR products were cloned into pGEM-T easy vectors and transformed into E.coli JM109.The positive recombinant plasmids identified by electrophoresis,PCR,and EcoR ivcleavage were sequenced.The nucleotide and were obtained by comparing with the ful-llength sequence of the other reference strains.Potential B-cell epitopes of VP1 were predicted and epitope peptide segments were synthesized.They were ident ified by indirect ELISA.The result showed that VP1a and VP1d were predominant B-cell epitope of VP1,which provided valuable information for further study on the vaccine of FMD.

Key words: FMDV, Structural protein VP1, Indirect ELISA, B-cell epitope

CLC Number:

ZHANG Yu, WANG Yong-lu, ZHANG Yong-guang, FANG Yu-zhen, PAN Li, JIANG Shou-tian, LU Jian-liang, LIU Li-kuan, ZHANG Zhong-wang, ZHANG Shu-gang, LI Zheng-feng, DU Jin-xin. Screening and Identification of B Cell Epitopes of VP1 of Foot-and-mouth Disease Virus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(5): 81-85. doi: 10.7668/hbnxb.2009.05.018.

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http://www.hbnxb.net/EN/Y2009/V24/I5/81

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