Preparation of Monoclonal Antibodies against Glutathione-S-transferase(GST)

doi:10.7668/hbnxb.2011.04.014

Abstract

Abstract: The monoclonal antibodies(McAbs) against Glutathione-S-transferase(GST) were prepared in the present work.Balb/C mice were immunized with purified GST and the cell fusion was performed by standard method.The hybridom secreting antibodies were subcloned three times by limiting dilution.Two hybridomas,which were named as 4B7-E4 or 4B7-F4 and secreted McAbs against GST,were picked out by indirect ELISA.The ELISA antibody titers of cell culture supernatant and ascite of 4B7-E4 and 4B7-F4 were 1∶ 4 000 or 1∶ 3 000,and 1∶ 3×10⁶ or 1∶ 2×10⁶ respectively.Western-blot showed that these.McAbs can specifically react with GST and recombinant GST.

Key words: GST, McAb, Indirect ELISA

CLC Number:

TENG Man, LUO Jun, WANG Qiu-xia, WANG Li, ZHANG Gai-ping. Preparation of Monoclonal Antibodies against Glutathione-S-transferase(GST)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(4): 82-85. doi: 10.7668/hbnxb.2011.04.014.

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