Sulfur-selenium-tungsten Compete the Molybdate Uptake by Modifying the Gene Expression of Predicted Molybdate Transporters

doi:10.7668/hbnxb.2015.05.036

Abstract

Abstract: Our objective was to explore the effects of sulfur (S),selenium (Se),and tungsten (W) application on growth and molybdenum (Mo) concentrations of oilseed rape and predicted molybdate transporter gene expression were analyzed using hydroponics.The dry weight and shoot S concentration were decreased with S-deficiency,but the Mo concentration was increased slightly.However,the Mo concentration showed no effect with supplied Se,while the Mo level showed lower Mo levels with W treatment.The S concentration was not affect by Mo deficiency.In addition,expression levels of Arabidopsis thaliana MOT1 and MOT2 homologous genes in all treatments were reported and indicate Bra007781, Bol020963,and Bra011159 had a higher expression levels than Bol001439 and Bal035189 in both shoot and root.And the Bra007781, Bol020963,and Bra011159 gene were expressed mainly in shoot. Bol001439 and Bal035189 were poorly expressed except root under Mo-deficient conditions. Bra007781 and Bol001439 were peculated the high-affinity molybdate transporter,and Bol020963 and Bra011159 were peculated the low-affinity molybdate transporter. Bra035189 was not taken into transport process. ST5 ; 1 was speculated a co-transporter in sulfate and molybdate transporter.In conclusion,competing ions (S,Se,and W) application had a marked impact on Mo distribution and accumulation,which was the first investigation of effect of S,Se,and W on Mo transporter homologous genes expression of rapeseed.

Key words: Molybdenum, Homologous genes, Sulfur, Selenium, Tungsten, Brassica napus

CLC Number:

S143.7

QIN Shi-yu, SUN Xue-cheng, HU Cheng-xiao, TAN Qi-ling. Sulfur-selenium-tungsten Compete the Molybdate Uptake by Modifying the Gene Expression of Predicted Molybdate Transporters[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(5): 232-238. doi: 10.7668/hbnxb.2015.05.036.

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