Spatial and Temporal Accumulation Characteristics of Cadmium in Sweetpotato Which Grown in Cadmium Contaminated Soil

doi:10.7668/hbnxb.20191671

Abstract

Abstract: In order to further clarify the spatial and temporal accumulation characteristics of cadmium in sweetpotato and to explore the causes of low cadmium accumulation in fleshed storage root,the temporal and spatial differences of cadmium accumulation in Xiangshu 20 were detected by atomic absorption spectrometry(AAS)in this study,and the expression difference of homologous genes related to cadmium accumulation in sweetpotato were analyzed by Fluorescence quantitative PCR.The results showed that the cadmium accumulation of sweetpotato followed the characteristics of root>stem>leaf. When plants were growth in the soil with 0.6 mg/kg cadmium,the increase rate of cadmium concentration in sweetpotato range from 3.05 to 8.75,and when plants growth in the soil with 2.0 mg/kg cadmium,the increase rate of cadmium concentration in sweetpotato range from 6.25 to 26.89. The accumulation rate of cadmium in shoots and leaves was higher than that in fleshed storage roots. The cadmium accumulated in leaves was not easy to transfer again,so cadmium concentration in new leaves was lower than that in old leaves. The homologous genes related to plant cadmium accumulation and transport existed in sweetpotato genome,and most of them had multiple homologous copies. Although these genes were induced to express with the increase of cadmium accumulation in plants,the spatiotemporal expression patterns of these genes were different. Compared with the expression pattern of genes related to cadmium accumulation,the expression of cadmium stress response related genes was been induced by cadmium accumulation more obvious. The cadmium accumulation in sweetpotato increased with the increase of soil cadmium concentration,and increased with the extension of growth time. The accumulation of cadmium in fleshed storage roots was lower than that in peels,stems and leaves,and the accumulation rate of cadmium in fleshed storage roots were much lower than that in shoots with the extension of growth time. The homologous genes related to plant cadmium accumulation and transport existed in sweetpotato genome,and most of them had multiple homologous copies. The spatiotemporal expression patterns of these genes were different,it was suggested that this expression difference was related to the cadmium accumulation characteristics of sweetpotato.

Key words: Sweetpotato, Cadmium accumulation, Homologous genes, Spatial and temporal expression patterns

CLC Number:

S143.7

ZHANG Daowei, DONG Fang, ZHANG Ya, HUANG Yanlan, ZHANG Chaofan. Spatial and Temporal Accumulation Characteristics of Cadmium in Sweetpotato Which Grown in Cadmium Contaminated Soil[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 289-298. doi: 10.7668/hbnxb.20191671.

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