Effects of Exogenous Abscisic Acid on Membrane Permeability and Antioxidant System of Sweetpotato at Seedling Stage under Drought Stress

doi:10.7668/hbnxb.2018.02.025

Abstract

Abstract: In order to investigate the effects of exogenous ABA on the resistance to drought stress at seedling stage in sweetpotato, two sweetpotato cultivars(JS21,a drought-tolerant cultivar,and JZ1,a drought-sensitive cultivar) were used to investigate the effects of exogenous abscisic acid(ABA) on protection against drought damage as well as on membrane permeability and antioxidant defense systems of sweetpotato at seedlings stage under drought stress. The results showed that after 12 days drought stress(AW),the relative electrical conductivity in leaves and the malondialdehyde(MDA) content in leaves and roots of drought stress treatment(PEGW) were higher than those of the control for both JZ1 and JS21,while the root activities were lower than those of the control. The activities of superoxide dismutase(SOD),peroxidase(POD) and ascorbate peroxidase(APX) in leaves and roots in JS21(JS21-PEGW) were significantly higher than those of the control(JS21-C),while glutathione reductase(GR) in leaves and ascorbate peroxidase(APX) in roots in JZ1(JZ1-PEGW) were lower than those of the control(JZ1-C). The antioxidant enzymes activities in leaves and roots and the root activities in drought-tolerant cultivar JS21(JS21-PEGW) were significantly higher than those of drought-sensitive cultivar JZ1(JZ1-PEGW),while the MDA content in leaves and roots were significantly lower than those of JZ1(JZ1-PEGW). After 12 days drought stress(AW),application of exogenous ABA significantly inhibited the relative electrical conductivity in the leaves of seedlings,reduced the accumulation of MDA in leaves and roots. Additionally,exogenous ABA significantly increased root activity and caused significant increases in SOD,POD,CAT,APX and GR activities in leaves and roots in both cultivars under drought stress. In conclusion,the results suggested that ABA played an important role in the tolerance of sweetpotato against drought stress resulted from the alleviation of membrane damage as indicated by decreases of the relative electrical conductivity and MDA content,enhanced root absorption through increases of root activity,as well as through ROS scavenging due to increase antioxidant defense systems capacity.

Key words: Abscisic acid, Antioxidant system, Drought stress, Membrane permeability, Sweetpotato

CLC Number:

S531.01

ZHANG Haiyan, DUAN Wenxue, DONG Shunxu, XIE Beitao, WANG Baoqing, ZHANG Liming. Effects of Exogenous Abscisic Acid on Membrane Permeability and Antioxidant System of Sweetpotato at Seedling Stage under Drought Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(2): 177-187. doi: 10.7668/hbnxb.2018.02.025.

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