Cloning and Sequence Analysis of nsdAmgh Gene in Streptomyces roseoflavus Men-myco-93-63

doi:10.7668/hbnxb.2009.05.017

Abstract

Abstract: nsdA (Negative regulator of Streptomyces differentiation) is globally negative regulator gene first found in Streptomyces coelicolor.In this study,nsdA gene of Streptomyces roseoflavus strains Men-myco-93-63 was amplified using the primer sequences designed from.Streptomyces coelicolor A3(2).After sequencing,Blastn,and Blastx,it showed that the cloned fragment from Streptomyces roseoflavus Men-myco-93-63 with the primer of nsdA-2R and nsdA-2L compairing with reported nsdA conservative nucleotide sequence homology on the region reached 99%.The fulllength gene sequence of Men-myco-93-63 nsdA gene amplification with primers of nsdA WZ-R and nsdA WZ-L had a complete open reading frame encoding 369 amino acids,with the Streptomyces lividans and Streptomyces coelicolor A3(2) nsdA gene at nucleotide sequence 100%,and amino acid sequence homology up to 99%.The fulllength nsdA gene of Streptomyces roseoflavus Men-myco-93-63 was named nsdA_mgh,which will promote the obtaintion of high-yielding antibiotic strains of Streptomyces roseoflavus Men-myco-93-63 through interrupting the negative regulator.

Key words: Streptomyces roseoflavus Men-myco-93-63, nsdA gene, Cloning, Sequence analysis

CLC Number:

Q785

SHEN Feng-ying, LIU Li-qiang, WU Wei-gang, LI Ya-ning, LIU Da-qun. Cloning and Sequence Analysis of nsdA_mgh Gene in Streptomyces roseoflavus Men-myco-93-63[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(5): 77-80. doi: 10.7668/hbnxb.2009.05.017.

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