Cloning and Sequence Analysis of β-xylanase Structural Gene and the 5′Flanking Regions Gene from Aspergillus niger

doi:10.7668/hbnxb.2009.05.016

Abstract

Abstract: In this paper,the cloning of a β-xylanase structural gene and its 5′flanking regions gene from Aspergillus niger was studied.On the base of nucleic acid DNA sequence of β-xylanase gene,a pair of degenerate primers was designed.Using the genomic DNA of Aspergillus niger as template,a 1.6 kb fragment was amplified by PCR and cloned into the vector pBS-T.Sequence analysis showed that the structural gene of β-xylanase is 744 bp length including a 66 bp intron and a 678 bp encoding region,which encoded a polypeptide of 225 amino acids and included the signal sequence of 18 amino acids.The homology analysis of the xynB cDNA sequence shares 98% in base sequence with the β-xylanase gene in GeneBank and the amino acid sequence shares 99%.Upstream of the tranlation start site,there were classic core element of Eukaryotic Gene Promoter,such as transcription start site,TATA box and CAAT box elements.The work was beneficial in construction of homologus expression for xylanase efficient expression vector.

Key words: Aspergillus niger, Xylanase, Gene cloning, Sequence analysis

CLC Number:

TQ926.3

BAI Ai-zhi, YAN Zu-wei, TANG Guo-min, LIANG Yun-zhang. Cloning and Sequence Analysis of β-xylanase Structural Gene and the 5′Flanking Regions Gene from Aspergillus niger[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(5): 73-76. doi: 10.7668/hbnxb.2009.05.016.

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