Effect of Staphyloccocus aureus on TLR2 Expression in Mammary Glands of Small-tail Han Sheep

doi:10.7668/hbnxb.2016.05.008

Abstract

Abstract: Toll-like receptor (TLR) family plays a key role in innate immunity and various inflammatory responses,for recognizing the bacterial pathogen and mediated the signal path of inflammation.To investigate the expressions of TLR2 in mammary glands of Small-tail han sheep were infected with Staphylococcus aureu, and to explore the mechanism of TLR2 in mastitis,the qRT-PCR and IHC technology was used to detect the expression of TLR2 mRNA and TLR2 protein in the mammary gland of control and experimental groups.The results showed that the TLR2 mRNAs and protein were expressed in all mammary glands of Small-tail han sheep in control and experimental groups.The levels of TLR2 mRNA and protein was lowest in control group,was highest at 48 h after Staphylococcus aureu infusion,significantly higher than that of control and 96 h group(P<0.05).After infected 96 h,the levels of TLR2 mRNA and protein was significantly reduced than that of 48 h group(P<0.05).The protein of TLR2 were located in the epithelial cell of mammary gland acinus,were expressed in epithelial cell of mammary gland acinus dropped and inflammatory cells as lymphocytes after effected Staphylococcus aureu.The results implied that the expression of TLR2 in the epithelial cell of mammary gland was up-regulated to recognize pathogenic bacteria,arouse the innate immunity.

Key words: Staphylococcus aureu, Small-tail han sheep, Mastitis, Toll-like receptors 2(TLR2)

CLC Number:

MA Xiaojun, CHEN Fuqiang, ZHANG Xiaoli, LI Fadi, TANG Ran, ZHANG Chen, SONG Xiaoyu, ZHANG Xin. Effect of Staphyloccocus aureus on TLR2 Expression in Mammary Glands of Small-tail Han Sheep[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(5): 50-55. doi: 10.7668/hbnxb.2016.05.008.

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