Analysis of Abiotic Stress Resistance Ability of Transgenic Tobacco Expressing GmPRP and SbPRP from Soybean

doi:10.7668/hbnxb.2016.05.009

Abstract

Abstract: In order to realize the application of PRPs genes in plant genetic engineering,the GmPRP and SbPRP from soybean were cloned into plant expression vector of pRI101,respectively,and then introduced into tobacco by Agrobacterium-mediated transformation.Two positive transgenic tobacco plants of GmPRP and four positive transgenic tobacco plants of SbPRP were obtained.The transgenic tobacco plants were treated with salt,drought and cold stresses.The results showed that the contents of proline and soluble sugar in SbPRP transgenic plants were significant higher than wild-type plants and the contents of malondialdehyde in SbPRP transgenic plants were significant lower than wild-type plants under salt stress.While the contents of proline,soluble sugar,and malondialdehyde in GmPRP and SbPRP transgenic plants had no significant differences compared to wild-type plants under drought stress.The contents of proline in GmPRP and SbPRP transgenic plants were both significant higher than wild-type plants and the contents of malondialdehyde in GmPRP and SbPRP transgenic plants were both lower than wild-type plants under cold stress.These data indicated that the transgenic tobacco plants constitutively expressing SbPRP showed an increased tolerance to salt and cold stresses and the transgenic tobacco plants constitutively expressing GmPRP showed an increased tolerance to cold stress,which may provide a way toward the application of them.

Key words: Soybean, Proline-rich protein, Tobacco, Stress resistance

CLC Number:

Q78
S565.03

ZHAI Ying, ZHANG Jun, ZHAO Yan. Analysis of Abiotic Stress Resistance Ability of Transgenic Tobacco Expressing GmPRP and SbPRP from Soybean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(5): 56-61. doi: 10.7668/hbnxb.2016.05.009.

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