Abstract: Using P1 and P2 as primers and extracted Avian encephalomyelitis virus total as template,810 bp VP1 gene fragment was amplified by RT-PCR. VP1 gene fragment was cloned into pMD18-T vector,The positive plasmid contained VP1 gene was determinded by restriction analysis and PCR. Then using P3 and P4 as primers and plasmid pMD18-T-VP1 as template,VP1 gene fragment was obtained by PCR amplication. then,VP1 gene and plant expression vector pBI121 were digested with Sma I and Sac I,extracted,connected,recombinant plasmid was transformed into agrobacterium tumefaciens EHA105 by freeze-thawing method,recombinant plasmid pBI121-VP1 was successfully constructed by digested and sequenced

Key words: Avian encephalomyelitis virus, VP1 gene, pBI121 plant expression vector

CLC Number: 

• Q78