Cloning and Characterization of STK Class Disease Resistance Gene Homology Sequences from Wheat

doi:10.7668/hbnxb.2010.05.004

Abstract

Abstract: The degenerated primers were designed based on the conserved amino acid sequences of the catalytic domainⅠand Ⅷ of the Serine /Threonine(STK) protein kinase-like proteins,and six open-reading of disease resistance gene analogs(RGAs) named Lr19-RGA1,Lr19-RGA2,Lr19-RGA3,Lr35-RGA1,Lr35-RGA2 and TC-RGA were obtained by PCR using cDNA as templates extracted from wheat TcLr19,TcLr35 and Thatcher. All catalytic domainsⅡ-Ⅷ of STK protein kinase were found in the deduced amino acid of Lr19-RGA1,Lr19-RGA2,Lr19-RGA3,Lr35-RGA1 and Lr35-RGA2 after aligned in the NCBI with BLASTp. Homology analysis indicated that they all have different homologies to STK resistance genes that have cloned. These results provide the basis for further study on cloning the STK resistance genes in wheat.

Key words: Wheat, Leaf rust, Serine-threonine kinase( STK), Resistance gene analogs

CLC Number:

Q94.8

ZHANG Nan, WANG Haiyan, LIU Daqun. Cloning and Characterization of STK Class Disease Resistance Gene Homology Sequences from Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(5): 20-24. doi: 10.7668/hbnxb.2010.05.004.

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