Co-transformation of Puccinellia chinampoensis PuCAT Gene and Bar Gene into Alfalfa with Agrobacterium-mediated Method

doi:10.7668/hbnxb.2017.S1.001

Abstract

Abstract: In order to improve the salt and alkali tolerance of alfalfa,the plant expression vector p3300-35S-PuCAT -NOS was constructed and transformed into alfalfa via Agrobacterium mediated. The selectable marker gene was Bar gene. And the genetic transformation system was optimized,including:pre-incubation time,bacteria concentration,inoculation time,co-cultivation time and antibiotic concentration. The transformed plants were selected with glufosinate ammonium. 30 transformed plants were identified as positive in the gene expression by PCR and RT-PCR which suggested that the PuCAT gene had been integrated into the genome of the regenerated plants.

Key words: Alfalfa, PuCAT gene, Transgenic plants, Agrobacterium mediated, Salt and alkali stress

CLC Number:

Q78
S551.03

REN Wei, WANG Jiaying, REN Huahua, WANG Yingzhe, WANG Menghan, XU Ankai. Co-transformation of Puccinellia chinampoensis PuCAT Gene and Bar Gene into Alfalfa with Agrobacterium-mediated Method[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(S1): 1-6. doi: 10.7668/hbnxb.2017.S1.001.

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