Physiological Responses to Chilling Stress in Transplastomic Tobacco with PuP5CS Gene

doi:10.3969/j.issn.1000-7091.2012.03.036

Abstract

Abstract: To identify the responses to Chilling Stress in transplastomic tobacco with PuP5CS gene，sense and?antisense plant expression vector of PuP5CS gene were constructed and introduced into tobacco via Agrobacterium?tumefaciens. For the pBI121-PuP5CS + transgenic plants，the levels of soluble suger，proline，chlorophyll a and chlorophyll b were significantly higher than wild-type tobacco，the level of malondialdehyde(MDA)was significantly?lower than wild-type under the chilling stress，improved it's cold resistance. But for the pBI121-PuP5CS-transgenic?plants，the levels of them were not significant.?

Key words: P5CS, Transgenic plants, Tobacco, Chilling stress

CLC Number:

S572

XU Bo, REN Wei, XU An-kai, WANG Zhi-feng, SUN Qi-zhong. Physiological Responses to Chilling Stress in Transplastomic Tobacco with PuP5CS Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 186-190. doi: 10.3969/j.issn.1000-7091.2012.03.036.

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http://www.hbnxb.net/EN/Y2012/V27/I3/186

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