The Effects of Intercropping Green Manure on Functional Diversity of Microbial Community in Tobacco Planting Soil

doi:10.7668/hbnxb.201751398

Abstract

Abstract: The effects of green manure intercropping on the functional diversity of soil microbial communities in tobacco growing soils were studied by Biolog technique to provide the theoretical basis for application of green manure intercropping with tobacco.The average well color development (AWCD) of soil microbial carbon metabolic activity at the stages of rosette and maturity were intercropped with Melilotus officinali > Lolium > control, indicating that intercropping with green manure had a significant effect on soil microbial carbon utilization. The Shannon index,Simpson index and Mclntosh index of intercropping green manure were higher than those of control, while the soil microbial Shannon index, Simpson index and Mclntosh index of intercropping Melilotus officinalis had the highest value. The results showed that intercropping green manure affected the microbial community structure of tobacco growing soil and improved the microbial community diversity of tobacco growing soil. Metabolic intensity analysis of different carbon sources showed that the utilization of amino acid, carboxylic acid and carbohydrate by soil microorganisms in different green manure and different growth stages was significantly higher than that of ammonia/amine, miscellaneou and polymer carbon sources. Principal component analysis showed that the functional diversity of soil microbial community was significantly different, and carboxylic acids, amino acids and sugars were the three main carbon sources, which led to the difference in soil microbial metabolism. Intercropping green manure enhances the ability of soil microorganism to utilize carbon source, and improves the diversity of soil microbial community structure and community function in tobacco growing soils. The effect of intercropping Melilotus officinalis is more obvious.

Key words: Tobacco planting soil, Microbial community, Biolog Eco board, Green manure intercropping

CLC Number:

S551

ZHAO Dongxue, WANG Panpan, CHANG Chunli, LI Jinqiu, WANG Jingying, AO Hong. The Effects of Intercropping Green Manure on Functional Diversity of Microbial Community in Tobacco Planting Soil[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(5): 201-207. doi: 10.7668/hbnxb.201751398.

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