Study on Soil Bacterial Community in Stipa baicalensis Steppe

doi:10.7668/hbnxb.2010.03.014

Abstract

Abstract: This study compared the bacterial diversity from soil in Stipa baicalensis steppe by polymerase chain reaction-denaturing gradient gel electrophoresis( PCR-DGGE) with four different DNA extraction methods.The DNArecover efficiency of the kit method was higher than others; further more the quality of DNA from kit is much better through analysis of the DGGE bands and sequencing the bands.The results suggested the microbial community in Stipa baicalensis steppe soil was mainly consisted of Actinobacteria,Proteobacteria,Bacteroidetes,Gemmatimonadetes, Acidobacteria,Verrucomicrobia．

Key words: Stipa baicalensis steppes, Microbial community, DNA extraction, PCR-DGGE

CLC Number:

S154.36

ZHANG Yong-sheng, LAI Xin, ZHANG Jing-ni, LI Gang, ZHAO Shuai, YANG Dian-lin. Study on Soil Bacterial Community in Stipa baicalensis Steppe[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(3): 58-63. doi: 10.7668/hbnxb.2010.03.014.

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http://www.hbnxb.net/EN/Y2010/V25/I3/58

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