Research on DNA Extraction Methods from a Single Nematode

doi:10.7668/hbnxb.2014.02.024

Abstract

Abstract: To explore an efficient, stable, low-cost DNA extraction method from single nematode for the support of molecular identification of nematodes, using six species of plant-parasitic nematodes as experimental materials, four DNA extraction methods, namely, direct lysis method, freeze-thaw lysis method, cutting lysis method, freeze-thaw cutting lysis method were designed and compared in order to check their efficiency of DNA extraction from single nematode. The results showed that cutting lysis method and freeze-thaw cutting lysis method were the most efficient and stable methods for DNA extraction of single nematode, then the freeze-thaw lysis method, and the direct lysis method was the worst one. Total DNA with high-quality from a single nematode could be gained efficiently and stab- ly through two methods, namely, cutting lysis method and freeze-thaw cutting lysis method. And the extracted DNA meeted requirement of the following PCR amplification.

Key words: Proteinase K, Lysis, Freeze-thaw, Nematode-cutting in tube, DNA extraction

CLC Number:

S154.38+6

RONG Wan-tao, WANG Jin-cheng, LIU Peng, LIN Yu, GUO Jing-ze, HUANG Guo-ming, SUN Jian-hua. Research on DNA Extraction Methods from a Single Nematode[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(2): 127-132. doi: 10.7668/hbnxb.2014.02.024.

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