Development of EST-SSR Primers and Cluster Analysis of Some Cultivars in Grape

doi:10.7668/hbnxb.2014.02.023

Abstract

Abstract: To develop the new EST-SSR marks from the EST-SSR database of grape germplasm, the statistic software of SSRs were utilized to search the sequences of EST containing SSRs, then the available were selected and the corresponding primers were designed and filtered. All the obtained 16 pairs of pleomorphic primers were used to carry out PCR amplification for 62 grape materials. Using UPGMA method to construct dendrogram based on the similarity coefficient calculated by the software of NTSYS. To invstigate the genetic relationships of grape germplasm resources and farther validate the feasibility of EST-SSR on classifying grape germplasm resources, the clustered re- sult was analyzed. The result indicated that the similarity coefficient of majority germplasm was more than 0. 74, and 62 grape materials were classified into 4 groups at 0. 658. Most grape germplasm resources having close kinship were clustered together, and the similarity coefficient of intraspecies was bigger than interspecies. The result was consist- ent with that acquired from traditional taxonomy.

Key words: Grape, EST-SSR, Cluster analysis

CLC Number:

S663.03

WANG Juan, TAO Yong-huan, SONG Shang-wei. Development of EST-SSR Primers and Cluster Analysis of Some Cultivars in Grape[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(2): 121-126. doi: 10.7668/hbnxb.2014.02.023.

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