Rapid Extraction of Genome DNA from Soybean Dry Seed for SSR Analysis

doi:10.3321/j.issn:1000-7091.2007.02.032

Abstract

Abstract: To develop a rapid DNA extraction method, the quality of DNA was examined with soybean dry seeds as materials, adding proteinase K, NP-40 and Tween-20 to SDS extracting solution, and reducing the chloroform/isoamyl extraction times. The results showed that when adding the three reagents to SDS extracting solution, the OD₂₆₀/OD₂₈₀ value of DNA was between 1.8 and 2.0 even extracted once by chloroform/isoamyl. Thus, good quality and quantity template DNA suitable for SSR analysis was obtained with reduced time.

Key words: Soybean, DNA extraction, SDS, SSR

CLC Number:

S565.1

ZHANG Wei, XIE Fu-ti, SONG Xian-jun, CAO Ping, GUO Yu-hua. Rapid Extraction of Genome DNA from Soybean Dry Seed for SSR Analysis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(2): 133-135. doi: 10.3321/j.issn:1000-7091.2007.02.032.

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http://www.hbnxb.net/EN/Y2007/V22/I2/133

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