The Influence of the DifferentR ice Tissues TotalDNA in ChloroplastMarkersAnalysis

doi:10.7668/hbnxb.2010.S2.007

Abstract

Abstract: "Chloroplast DNA marker is a common method of rice molecular analysis.However extract rice chloroplast DNA is a complex and time-consuming process.In order to simplify the steps of analyzing,this study use the CTAB and SDS methods to extract the total DNA(cultivation rice indica 93-11 and japonica SN265) from three different tissues of rice,including leafs roots and seeds,then amplify the total DNA templates with three pairs of chloroplast gene interval area markers(psbA-trnH,petG-trnP and infA-rpl36) and two pairs of chloroplast Indica-japonica classification markers(ORF100 and ORF29-TrnCGCA).The amplification results of roots and seeds are consistent with the leaf results and are also in expectation.And then use sixty weedy rice seed total DNA templates with chloroplast marks of Indica-japonica classification in PCR reaction.The results show these groups of chloroplast markers can amplify the target fragment from weedy rice seed total DNA and do Indica-japonica classification effectively.This conclusion can simplify the analysis process with rice chloroplast DNA markers and accelerate the analysis speed. "

Key words: Rice, DNA extraction, ChloroplastDNA marker, PCR amplification

CLC Number:

S511.03

ZHOU Suo-tong, LIU Zhi-wen. The Influence of the DifferentR ice Tissues TotalDNA in ChloroplastMarkersAnalysis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(S2): 27-30. doi: 10.7668/hbnxb.2010.S2.007.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2010.S2.007

http://www.hbnxb.net/EN/Y2010/V25/IS2/27

[1]	ZHOU Xinqiao, CHEN Dagang, SHAN Zelin, GUO Jie, CHEN Pingli, CHEN Ke, LIU Chuanguang, CHEN Youding. Study on Dynamic Plant Type Characteristics of Different Double Cropping Super Rice in South China [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 68-77.
[2]	LI Xia, LUO Lihui, ZHOU Ya, YANG Dingqing, WANG Peng, LI Sen. Effects of Straw Returning on Soil Active Organic Carbon Components and Enzyme Activities in Rice-Rape Rotation [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 124-131.
[3]	WANG Ya, WANG Yuetao, SHEN Guanwang, WANG Fuhua, WANG Shengxuan, BAI Tao, YIN Haiqing. Improvement of Rice Blast Resistance by Pyramiding the R Gene Pigm and the Non-R Gene bsr-d1 [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 157-165.
[4]	WANG Yanning, HUANG Tao, WU Guangliang, HUANG Shiying, ZHONG Qi, WANG Peng, YANG Mengmeng, LI Caijing, CHENG Qin, HE Haohua, JIN Wei, GUO Ling, BIAN Jianmin. Transcriptome Analysis of Rice Bud Stage in Response to Cadmium Stress [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 25-35.
[5]	LI Wenxiu, DAI Li, WANG Lihuang, FANG Baohua, WANG Xuehua. Carbon and Nitrogen Metabolism and Yield Composition in Multi-panicle and Large-panicle Super Rice Varieties at Young Panicle Differentiation Stage [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 103-112.
[6]	SHI Ailong, WANG Lihuang, LI Wenxiu, WANG Xuehua. Effects of Water and Fertilizer on Agronomic Characters and Yield of Double-cropping Hybrid Rice [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 145-157.
[7]	HAN Zhenghong, DUAN Yuxuan, XU Shanbin, WANG Jingguo, LIU Hualong, YANG Luomiao, JIA Yan, XIN Wei, ZHENG Hongliang, ZOU Detang. *Improvement of Grain Shape in Rice by Knocking GS3* and GS9 via CRISPR/Cas9 System** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2): 9-17.
[8]	ZHOU Liping, ZHAO Qiu, ZHANG Xinjian, NING Xiaoguang, YUAN Liang, LI Yanting, ZHAO Bingqiang. Effect of New Type Synergistic Compound Fertilizer on Rice Nutrient Absorption and Yield [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2): 112-120.
[9]	LIANG Yugang, ZHOU Jing, YU Zhengjun, LI Jingyi, HUANG Huang, ZHAO Zhenghong. Effect of Rice-duck Farming on the Composition and Diversity of the Weed Community in Direct Seeding Rice Paddies [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2): 160-170.
[10]	LI Fujian, XU Dongyi, LIU Kaili, ZHU Min, LI Chunyan, ZHU Xinkai, DING Jinfeng, GUO Wenshan. Effects of Tillage Methods on Growth and Physiology of Seedlings Stem and Tillers and Their Productivity of Wheat in Rice Stubble [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 58-67.
[11]	LIU Junfeng, LI Yimeng, LIANG Chao, ZHOU Chanchan, WANG Shu, JIA Baoyan, HUANG Yuancai, WANG Yan, WANG Yun. Effect of Nitrogen Application Pattern and Row Spacing on Canopy Structure and Yield of Rice [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 77-85.
[12]	JING Lili, PENG Ting, ZHAO Yafan, ZHAO Shuaibing, WANG Tongtong, LI Yuan, CHENG Yuan, DU Yanxiu, ZHANG Jing, SUN Hongzheng, ZHAO Quanzhi. Regulatory Effects of Two Grain Filling Regulators on Inferior Spikelets Filling of Large Panicle Rice [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 86-94.
[13]	XU Lingqi, GUO Xiaohong, ZHANG Jianing, ZHAO Yang, LI Xiaolei, LIU Shaofeng, CUI Zhiyuan, AN Yiliang, LÜ Yandong. The Effect of Organic Fertilizer on the Quality of Dry Direct-seeding Rice [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 137-146.
[14]	CHEN Ziqi, WANG Weiping, ZHAO Hongqiang, WANG Hao, HAN Xiao, YANG Luomiao, XIN Wei, LIU Hualong, ZHENG Hongliang, WANG Jingguo. *QTL Analysis of Rice Cold Tolerance at Germination and Bud Stage Utilizing Indica-Japonica* RIL Population** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 50-57.
[15]	CHEN Yanqi, LIU Qiong, BIANBA Lamu, CHEN Songhe, ZHANG Heng, LI Chaosu, YANG Hongkun, ZHENG Ting, HUANG Xiulan, FAN Gaoqiong. Effects of Nitrogen Application Rate on Raw Grain Quality and Daqu Quality in Wheat-rice System [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 297-305.

Please choose a citation manager

Content to export