Associations Between the Insulin- like Growth Factor-1( IGF-1 ) Gene Polymorphism and BodyWeight in Taihe Silky Fowl

doi:10.7668/hbnxb.2010.S2.006

Abstract

Abstract: "Taihe silky fowl was used in this research to investigate the effect of IGF-1 as a candidate gene on chicken growth traits.primers for IGF-1was designed according to the database of chicken genomic sequence.PCR-SSCP and PCR-RFLP were used to detect the genotypes in order to discuss the relationship between genotypes and growth traits.One mutation was found in the exon 1 of IGF-1.The statistic results showed that 12 W body weight were significant different(P0.05)between different genotypes.AB genotype birds has significant higher(P 0.05)body weight on 12 W than AA genotype.Genetic variation of the 5' region of the IGF-1gene were detected by PCR-RELP,Three genotypes CC,CD and DD were identified after the PCR products were cut by Pst I restriction enzyme,the statistic results showed that 12 W body weight were not significant different between different genotypes"

Key words: Taihe silky fow, l IGF-1, Body weight

CLC Number:

S831

WU Yan-ping, HUO Jun-hong, XIE Ming-gui, LIU Lin-xiu, JI Hua-yuan, CHU Yi-shi, TANG Wei-guo, XIE Jin-fang. Associations Between the Insulin- like Growth Factor-1( IGF-1 ) Gene Polymorphism and BodyWeight in Taihe Silky Fowl[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(S2): 22-26. doi: 10.7668/hbnxb.2010.S2.006.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2010.S2.006

http://www.hbnxb.net/EN/Y2010/V25/IS2/22

References

[1] Salmon W D,Daughaday W H.A hormonally controlled serum factor which stimulates sulfate incorporation by cartilage in vitor[J].Journal of Laboratory and Clinical Medicine,1957,49:82-836.
[2] 陈照丽.IGF的系统学功能以及与肿瘤的关系[J].国外医学分子生物学分册,2003,25(1):34-37.
[3] 陈冰,刘晓辉.胰岛素样生长因子结合蛋白功能及其调节机制[J].生命化学,2001,21(5):384-386.
[4] Ballard F J,Johnson R J,Owens P C.Chicken insulin-like growth factor-1:Amino acid sequence,radioimmunoassay,and plasma level between strains and during growth[J].General and Comparative Endocrinology,1990,79:459-468.
[5] Rindderkneeht E,Humbel R E.The amino acid sequence of human insulin-like growth factor-Ⅰ and its structural homology with proinsulin[J].The journal of biological chemistry,1978,253(8):2769-2776.
[6] Robert M C,Timothy S H,Lain D C.Solution structure of human insulin-like growth factor-Ⅰ:a nuclear magnetic resonance and restrained noleeular dynamics study[J].Biochemistry,1991,30(22):5484-5491.
[7] Dawe S R,Francis G L,McNamara P J,et al.Purification,partial sequences and properties of chicken insulin-like growth factors[J].Journal of Endocrinology,1988,117(2):173-181.
[8] Klein A,Morrice D R,Sang H,et al.Genetic and physical mapping of the chicken IGF-1 gene to chromosome 1 and conservation of synteny with other vertebrate genomes[J].Journal of Heredity,1996,87:10-14.
[9] 王强.鸡IGF-1基因PCR-SSCP分析及其与肉用性状关系的研究[D].扬州:扬州大学,2006.
[10] Nagaraja S C,Aggrey S E,Yao J,et al.Trait association of a genetic marker near the IGF-1 gene in egg laying chickens[J].Heredity,2000,91:150-156.
[11] Seo D S,Yun J,Kang W,et al.Association of insulinlike growth factor-1(IGF-I)gene polymorphism with serum IGF-1 concentration and body weight in Korean Native Ogol chicken[J].Asian-Australasian Journal of Animal Sciences,2001,14(7):915-921.
[12] Kim M H,Seo D S,Ko Y.Relationship between egg productivity and insulin-like growth factor-1 genotypes in Korean Native Ogol chickens[J].Poultry Science,2004,83(7):1203-1208.
[13] 闵令江,潘庆杰,陈宏,等.寿光鸡IGF-J基因多态性与体重及屠体性状关系的研究[J].畜牧兽医学报,2005,36(7):645-648.
[14] 万秋蓓.鸡类胰岛素生长因子-1(IGF-1)基因多态性与蛋用性状关系及其表达的研究[D].扬州:扬州大学,2007.
[15] Amills M,N Jimemea,D Villalba,et al.Identification of three single nucleotide polymorphism s in the shicken insulin likegrowth factor 1 and 2 genes and their associations with growth and feeding traits[J].Poultry Science.2003,82(10):1486-1493.
[16] 孙汉,欧阳建华,王文君,等.鸡胰岛素样生长因子的研究进展[J].江西农业大学学报,2003,25(1):133-136.
[17] 沈华,王金玉,黄羽肉鸡IGF-1基因单核苷酸多态性与生长性状的相关研究[J].中国畜牧兽医,2006,33(10):58-60.
[18] Paul G Voorhoeve,Elisabeth F C van Rossuml.Association between an 1GF-1 gene polymorphism and body fatness:differences between generations[J].European Journal of Endocrinology,2006,154:379-388.

Please choose a citation manager

Content to export