The Spotted Chicken Newcastle Disease Virus Strain SX-1 of F Gene and HN Gene Co-Express in Eukaryotic Cell

doi:10.7668/hbnxb.2011.03.010

Abstract

Abstract: Based on The pG-F and pG-HN cloning plasmid templated in our lab,Newcastle disease virus strain SX-1 sequences of F gene and HN gene isolated from?spotted chicken were PCR amplified,15 amino acids as flexible short peptide Linker connected F and HN genetic fragments in vitro was inserted into pCI-neo eukaryotic expression vector to construct the co-expression vector pCI-F-HN of Newcastle disease virus strain SX-1 sequences of F gene and HN gene and transfected in Vero cells,and carried on expression of indirect immune fluorescence detection.The results showed that virus SX-1 strain F-HN combination of genes in Vero cells had high level expression and in fluorescence microscopy strong fluorescence signal could be observed,which suggested that F-HN combination protein had good reactionogenicity.It provids for further research efficient subunits genetic engineering?vaccine in foundation.

Key words: Spotted chicken newcastle disease virus, Strain SX-1 sequences, F-HN gene, Co-expression

CLC Number:

S831
Q782

LI Hong-li, ZHAN Li-e, ZHANG Wei-ye, TANG Juan, LU Bing-yang, WANG Cai-xian. The Spotted Chicken Newcastle Disease Virus Strain SX-1 of F Gene and HN Gene Co-Express in Eukaryotic Cell[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(3): 47-50. doi: 10.7668/hbnxb.2011.03.010.

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