Cloning of Celluase Gene cel6B from Lentinula edodes and Its Expression in Escherichia coil

doi:10.7668/hbnxb.2011.03.009

Abstract

Abstract: Total RNA was extracted from L.edodes cultured in liquid PDA medium.The predicted gene cel6B was cloned via RT-PCR and sequenced.The result of sequence analysis showed the gene belongs to Glycosyl hydrolases family 6.Prokaryotic expression vector pET28a-cel6B was constructed.The 46.4 kDa protein CEL6B was expressed based on the result of SDS-PAGE.The bacteria containing pET28a-cel6B was cultured in CMC-Na-congo red medium which has CMC-Na as the solo carbon source to test the cellulase activity.The result of primary bioactivity analysis showed that CEL6B has cellulase function.

Key words: Lentinula edodes, Cellulase, cel6B gene, Congo red, RT-PCR

CLC Number:

S67.3+9
Q78

YANG Ting, PEI Yan-xi, WANG Jing-xue. Cloning of Celluase Gene cel6B from Lentinula edodes and Its Expression in Escherichia coil[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(3): 42-46. doi: 10.7668/hbnxb.2011.03.009.

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