Acta Agriculturae Boreali-Sinica

ACTA AGRICULTURAE BOREALI-SINICA ›› 2011, Vol. 26 ›› Issue (3): 37-41. doi: 10.7668/hbnxb.2011.03.008

Special Issue: Animal husbandry； Biotechnology；

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Isolation, Identincation and Molecular Characterization of a H9N2 Isolate from a Broiler Flock Glandular Stomach

WANG You-ling1, YUAN Xiao-yuan2, XU Huai-ying2, YANG Jin-xing2, QIN Zhuo-ming2, LIAO Ming3   

  1. 1. The South China Agricultural University,Guangzhou 510642,China;
    2. Institute of Poultry Science,Shandong Academy of Agricultural Sciences,Jinan 250100,China
  • Received:2011-02-08 Published:2011-06-28

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Abstract: A newly H9N2 isolate named A/Chicken/Shandong/KD/09 was collected from a broiler flock glanduar stomach,The biological test showed that the isolate A/Chicken/Shandong/KD/09 exhibited the IVPI value with 0,and the isolate shared a lentogenic motif 335RSSR↓GL340 at the HA protein cleavage site,which indicated that A/Chicken/Shandong/KD/09 was lentogenic.The HA gene nucleotides(amino acids) homologies is 94.5%-100%(95.5%-99.8%) between the isolate A/Chicken/Shandong/KD/09 and the China H9N2 isolates.The HA gene nucleotides(amino acids) homologies is 80.9%-91.5%(88.1%-91.5%) between the isolate A/Chicken/Shandong/KD/09 and A/ck/Korea/ms96/96,A/Parakeet/chiba/1/97,A/turkey/Wisconsin/1/1966 H9N2 isolates.Phylogenetic analysis of HA gene showed that the A/Chicken/Shandong/KD/09 was similar to that of the A/chicken/Shandong/2/02.

Key words: H9N2 subtype avian influenza virus, Glandular stomach, Haemagglutinin gene

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Cite this article

WANG You-ling, YUAN Xiao-yuan, XU Huai-ying, YANG Jin-xing, QIN Zhuo-ming, LIAO Ming. Isolation, Identincation and Molecular Characterization of a H9N2 Isolate from a Broiler Flock Glandular Stomach[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(3): 37-41. doi: 10.7668/hbnxb.2011.03.008.

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