An Efficient Method for Genomic DNA Extraction from Mature Tissues of Blueberry(Vaccinium spp.)

doi:10.7668/hbnxb.2008.S2.047

Abstract

Abstract: To obtain high quality genomic DNA from mature tissues of blueberry( Vaccinium spp. ), a modif ied CTAB method was established.And then three different methods, the conventional CTAB method, rapid CTAB method and modi??fied CTAB method, were compared. The results showed that the modified method could obtain the expecting DNA thatreached the higher purity (OD260/OD280 is between 1. 75 and 1. 85) and higher yield ( 192 g/ g). Furthermore, the DNA can be digested completely with restriction endonuclease, and the electrophoresis bands of SRAP??PCR were clear and stable. It is concluded that this modified CTAB method should be suitable for extraction of genomic DNA from mature tissuesof blueberry( Vaccinium spp. ).

Key words: Blueberry( Vaccinium spp. ), Mature leaves, Genomic DNA extraction, Modified CTAB, SRAP

CLC Number:

S666.2

ZHANG Lu-jie, XIA Xiu-ying, XU Na, HE Jian-hua, XU Pin-san. An Efficient Method for Genomic DNA Extraction from Mature Tissues of Blueberry(Vaccinium spp.)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(S2): 205-208. doi: 10.7668/hbnxb.2008.S2.047.

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