Abstract: Bergenia purpurascens ( Hook． f． et Thoms． ) Eng1 ． is an endangered medicinal species mainly distributed in Yunnan， Sichuan and Tibet． There are a lot of reports about its chemistry and pharmacology but no reports on its genetic diversity and conservation． To reveal the genetic diversity of B. purpurascens in Yunnan， 18 genome DNA samples of B. purpurascens collected in Yunnan province were extracted from young leaves by modifiedCTAB methods and the screening of primers for ISSR analysis were conducted． The concentrations of DNA samplesvaried from 1 387． 5 ng / μL to 12 000 ng / μL while the ratios of A260 /A280were between 1 ． 61 and 1 ． 85． There wasonly one band on the electrophoretogram for each DNA sample． These results confirmed that the qualities of extracted DNA samples were good and the modified CTAB methods described in this paper could be used for extractingDNAs from plant materials with abundant polyphenol and protein． Twenty-two ISSR primers which gave good amplification results were screened out from 100 primers and this provided a solid basis for exploring genetic diversity ofB. purpurascens in Yunnan by ISSR molecular marker．

Key words: Bergenia purpurascens (Hook． f． et Thoms． ) Eng1 ． , Yunnan, DNA extraction, ISSR primer Screening

CLC Number: 

• S634