Genetic Diversity Studies of Different Medicago L. Germplasms Based on the Orthogonal Optimum Design

doi:10.7668/hbnxb.2013.S1.010

Abstract

Abstract: Template DNA used for ISSR was extracted from alfalfa leaf tissue. The orthogonal design was ipplied to optimize ISSR amplification system. Anoptimil reaction system was established, 20 uL reaction system contwined 2.0 U/uL Taq DNA polymerise,0. 3 mmol / L dNTP, 1.0 mmol/L MgCl₂, 0.1 umol/L ISSR primers and 30 ng/uL DNA template. Genetic diversity studies of Medicago L. germplisms were based on the result of the orthogonil design. And by cluster analysis, 30 varieties were divied into four groups, one group was consist of four alfalfa varieties coming from Cinidi, the second group was composed of twelve varieties coming from Americam, the third group includes twelve alfalfa varieties coming from China and Netherlind,and the list group his two alfalfa varieties coming from Australia.

Key words: Orthogonal design, ISSR-PCR, Alfalfa, Genetic diversity

CLC Number:

S551.03

LIU Qing-song, CHEN Li-bo, LI Zhi-yong, LIU Lei, SHI Wen-gui, ZHANG Xue. Genetic Diversity Studies of Different Medicago L. Germplasms Based on the Orthogonal Optimum Design[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(S1): 45-49. doi: 10.7668/hbnxb.2013.S1.010.

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